In the field of proteomics, the focus moves from identification to quantification. We are developing techniques to enable relative and absolute quantification of peptides and proteins. With MeCAT (Metal Coded Affinity Tagging) technology, quantitative proteomics can exploit the advantages of element mass spectrometry, such as high sensitivity and absolute quantification.
- Analysis and Quantification of modified DNA
The antitumor activity of numerous cytostatic drugs is predominantly based on the direct reaction of the substance with the DNA. To understand the mode of action of those drugs, elucidation of the key structures plays an important role. Based on these results, the next step is to quantify the formed products by means by elemental mass spectrometry.
Bdellovibrios are bacteria that hunt, invade and kill a variety of other bacteria. The unique lifestyle needs a specialised machinery for identifying suitable prey, attaching to it and entering the prey cell. We are especially interested in membrane constituents, such as membrane proteins and lipopolysaccharides and their role for the lifecycle of those unique predators.
- Gas Phase Behaviour of natural and artificial Nucleic Acids
The fragmentation behaviour of oligonucleotides, such as DNA, RNA and PNA (peptide nucleic acids), under different excitation conditions (CID, IRMPD, ECD, etc.) is of special interest. The non-covalent interaction of complementary and non-complementary oligonucleotide double strands in the gas phase will be studied using high resolution mass spectrometry.